Haploids by anther culture (Guha and Maheshwari)
Haploides por cultivo de anteras (Guha y Maheshwari)
- Date
- 1964 · confidence high
- Place
- University of Delhi, Delhi, India
- Credited to
- Sipra Guha and Satish C. Maheshwari
- Field
- Life & health
What was discovered
Guha and Maheshwari cultured Datura innoxia anthers in vitro and obtained haploid embryos from pollen. They showed that pollen grain cells can give rise to whole plants, opening the way to rapid fixation of homozygous lines.
The finding was published in Nature in 1964 (Guha and Maheshwari) from work in Delhi's Department of Botany; Bourgin and Nitsch obtained tobacco haploids in France in 1967.
What it led to
- doubled haploids in rice, wheat, rapeseed and barley breeding
- varieties obtained by anther culture in China from the 1970s
- microspore culture and androgenesis as a routine technique