Southern blot (DNA transfer)
Southern blot (transferencia de ADN)
- Date
- 1975 · confidence high
- Place
- University of Edinburgh, Edinburgh, Scotland, United Kingdom
- Credited to
- Edwin M. Southern
- Field
- Life & health
What it is
Southern transferred DNA separated by gel electrophoresis onto a membrane and detected it with a labelled probe. It allowed specific genome fragments to be identified and is the origin of DNA fingerprinting and molecular diagnostics.
What it led to
- DNA fingerprinting (Jeffreys, 1984)
- gene mapping and diagnosis of hereditary diseases
- sister techniques Northern and Western blot