Synthetic yeast chromosomes (Sc2.0)
Cromosomas sintéticos de levadura (Sc2.0)
- Date
- 2014-2017 · confidence medium
- Place
- NYU Grossman School of Medicine, New York, United States
- Credited to
- Jef Boeke and the international Sc2.0 consortium (with teams in Tianjin, Beijing and Shenzhen)
- Field
- Life & health
What it is
The Sc2.0 consortium designed and built artificial Saccharomyces cerevisiae chromosomes with reduced sequences, inducible recombination sites and no repetitive elements. The first (synIII) was published in 2014 and five more in 2017, with decisive contributions from Chinese teams.
The first synthetic eukaryotic chromosome (synIII) was completed by Boeke's team at Johns Hopkins (Annaluru et al., Science, 2014); the 2017 series was built largely in Chinese centres (Tianjin, Tsinghua, BGI) as well as US and British ones. Completion dates for the whole genome are approximate and still being updated.
What it led to
- consolidation of more than seven synthetic chromosomes in a single strain (2023)
- genome reshuffling in the laboratory (SCRaMbLE)
- directed evolution and whole-genome metabolic engineering platforms