Nanopore DNA sequencing (Oxford Nanopore MinION)
Secuenciación de ADN por nanoporos (Oxford Nanopore MinION)
- Date
- 1996-2014 · confidence high
- Place
- Oxford Nanopore Technologies, Oxford, England, United Kingdom
- Credited to
- John Kasianowicz, Daniel Branton, David Deamer, Mark Akeson and Oxford Nanopore (Gordon Sanghera, Clive Brown)
- Field
- Life & health
⚠️ Disputed: the sources disagree.
What it is
A DNA strand passes through a protein pore across a membrane and each base alters the ionic current in a characteristic way. Oxford Nanopore released the MinION, a phone-sized sequencer that reads very long fragments, in early access in 2014.
The idea of reading DNA through a nanopore comes from Kasianowicz, Branton and Deamer (1996) and Church; the patents and industrial development belong to Oxford Nanopore (founded in 2005 as an Oxford spin-out). The 2014 date is that of the MinION early-access programme; broad commercialisation followed in 2015.
What it led to
- on-site genomic sequencing during the Ebola epidemic in Guinea (2015) and Zika in Brazil
- complete assemblies of human chromosomes (telomere to telomere, 2022)
- direct RNA sequencing and detection of base modifications