Sanger DNA sequencing
Secuenciación de ADN de Sanger
- Date
- 1977 · confidence high
- Place
- MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
- Credited to
- Frederick Sanger (second Nobel Prize, 1980); Maxam and Gilbert (chemical method, Harvard)
- People
- 👤 Allan Maxam1942–👤 Frederick Sanger1918–2013👤 Walter Gilbert1932–
- Field
- Life & health
What it is
Chain-termination method using dideoxynucleotides to read the DNA base sequence. Automated in 1986, it made it possible to sequence the human genome.
Merged with «DNA sequencing by chain terminators» (duplicate, 23-09-2026).
What it led to
- The Human Genome Project
- The molecular genetics of disease
- Bioinformatics
- genomics
- the Human Genome Project
- genetic diagnosis
- next-generation sequencing (2005)
Built on
- DNA double-helix structure1953
- Restriction enzymes1968-1970
- structure of DNA
- DNA polymerase
- gel electrophoresis
Made possible
- Polymerase chain reaction (PCR)1983
- Automated fluorescent DNA sequencer (Hood and Applied Biosystems)1986-1987
- Human Genome Project1990-2003
- Next-generation sequencing (454, Solexa/Illumina)2005-2006
- CRISPR-Cas9 genome editing2012
- Nanopore DNA sequencing (Oxford Nanopore MinION)1996-2014
- automated sequencer (Applied Biosystems)
- Human Genome Project
- bioinformatics
- automated sequencers
- comparative genomics